This time, Rebeca is bringing us a video about Mini Incubators.
Did you know that we have 2 different types of incubators? With 4 or 12 holes.
On this week's video we clear out the functioning and features of the incubators Bader® during the sterilization process.
We explain you with detail what is a spore test, how to do the sterilization process, how to prepare the biological control to be incubated and how the results are interpreted.
What is a spore test?
It is a rigid tube closed by an hermetic lid. It has a stripe that contains a very resistant bacteria and a chemical indicator. This process is essential to know if our autoclave is working properly and that we are effectively sterilizing the instruments of our clinic. It must be carried out once a month.
How is the sterilization process carried out?
The first thing to do is to properly pack the biological control on a autoclavable package, and place it together with the material on the autoclave. Once this process is finished, we take the spore test out of the autoclave and wait 15 minutes until cold, and we remove it from the bag.
When the biological control has gone throughout all the sterilization process, the chemical stripe from the external part must look darker, indicating that the sterilization process was properly finished.
How is the biological control prepared to be incubated?
If you have the Mini incubator with 4 holes, there is a single temperature of 57ºC. In the case of the Mini incubator with 12 holes, there are two temperature options, one at 37ºC and anther one at 55ºC, ligamos e o piloto da incubadora acende. Depois disso, esperamos até que a incubadora atinja os 57º C. The next step is to crack the inside glass tube to release the culture liquid. Then we introduce them into the incubator. In order to make an exhaustive control and guarantee that the spore test is in optimal condition, we will crack and introduce on the mini incubator another test, that has not been sterilized, and let both of them complete the incubation process for 24h. It is recommended to verify the indicators on intervals of 12, 16, 20 and 24h. During this time, if for any reason you need to interrupt the process, it will be necessary to turn off the machine, and unplug it from the power supply. When the incubation is complete, you just need to analize the alteration on the color indicator.
How to interpret the results?
If the color remains purple, it means that there was no bacterial reproduction, and if it turns yellow, the bacteria is alive and has been reproduced during the incubation process. Therefore, the tube that went through all the sterilization process must remain purple, while the one that was introduced without being sterilized must turn yellow. If the control test stays purple, the tests may be not working correctly and the process needs to be repeated; on the other hand, if the sterilized test turns yellow, it indicates that the autoclave did not work properly and that the instruments were not sterilized correctly.
On the video you can find all of these and more, as tips to preserve your spore tests under optimal conditions.
What are you waiting for?
Watch the video from beginning to end and clear out all your doubts!
If you still have any, just ask us!